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ULK1 polyclonal antibody

货号: BS91404

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS91404

Product Name :ULK1 polyclonal antibody

Swiss-Prot :O75385

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB IHC

Application_all :WB:1:500-1:1,000 IHC:1:50-1:200

Background :Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity :ULK1 polyclonal antibody detects endogenous levels of ULK1 protein.

BiowMW :~ 150 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :PI3K AKT signaling,MTOR signaling,

Alternative Name :Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog (ATG1; Hatg1; Unc-51-like kinase 1; ULK1; KIAA0722

Immunogen :Synthetic peptide, corresponding to Human ULK1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ULK1 polyclonal antibody at 1:500 dilution LaneA:A549 whole cell lysate(40ug) LaneB:HEK293T whole cell lysate(40ug) LaneC:AML-12 whole cell lysate(40ug) LaneD:C6 whole cell lysate(40ug) LaneE:Panc1 whole cell lysate(40ug)

Immunohistochemical analysis of ULK1 staining in human breast cancer formalin fixed paraffin embedded tissue section.