Product Name :S100β polyclonal antibody
Reactivity :Human, Mouse, Rat, Goat, zebrafish
Applications :WB IF IP IHC
Application_all :WB:1:500-1:2000
IF:1:50-1:200
IHC:1:50-1:200
IP:1:50-1:200
Background :The family of EF-hand type Ca2<sup>+</sup>-binding proteins includes calbindin (previously designated vitamin D-dependent Ca2<sup>+</sup>-binding protein), S-100 α and β, calgranulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins), and the parvalbumin family members, including parvalbumin α and parvalbuminβ(also designated oncomodulin). The S-100 protein is involved in the regulation of cellular processes such as cell cycle progression and differentiation. Research also indicates that the S-100 protein may function in the activation of Ca2<sup>+</sup> induced Ca2<sup>+</sup> release, inhibition of microtubule assembly and inhibition of protein kinase C mediated phosphorylation. Two S-100 subunits, sharing 60% sequence identity, have been described as S-100 α chain and S-100 β chain. Three S-100 dimeric forms have been characterized, differing in their subunit composition of either two α chains, two β chains or one α and one β chain. S-100 localizes to the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. S-100 is also detected in almost all benign naevi, malignant melanocytic tumours and in Langerhans cells in the skin. Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues, and calbindin is present in intestine and kidney.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity :S100β polyclonal antibody detects endogenous levels of S100β protein.
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :NEF; S100; S100-B; S100beta; S100B; S100 beta
Immunogen :Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100B (NP_006263.1).
Modification :Unmodification
