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OGT polyclonal antibody

货号: BS90983

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS90983

Product Name :OGT polyclonal antibody

Swiss-Prot :O15294

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB: 1:500~1:2000

Background :O-linked N-acetylglucosamine (O-GlcNAc) transferase (also designated OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. O-GlcNAc transferase has been purified from rat liver. It exists as a heterotrimeric complex with two subunits of the same molecular mass and one shorter subunit. Both polypeptides are related; the short subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity :OGT polyclonal antibody detects endogenous levels of OGT protein.

BiowMW :~ 117 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :OGT; HINCUT-1; HRNT1; O-GLCNAC; MRX106; OGT1

Immunogen :Recombinant fusion protein containing a sequence corresponding to amino acids 857-1036 of human OGT (NP_858059.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using OGT antibody at 1:1000 dilution.

免疫沉淀（IP）

免疫组织化学（IHC）

免疫荧光（IF）