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CRISPR-Cas9 SP polyclonal antibody

货号: BS90331

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS90331

Product Name :CRISPR-Cas9 SP polyclonal antibody

Swiss-Prot :Q99ZW2

Host :Rabbit

Reactivity :Recombinant Cas9

Applications :WB

Application_all :WB: 1:500~1:1000

Background :CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity :CRISPR-Cas9 SP polyclonal antibody detects endogenous levels of CRISPR-Cas9 SP protein.

BiowMW :~ 160 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; csn1; SpyCas9

Immunogen :Synthetic peptide, corresponding to amino acids 750-800 of CRISPR/Cas9.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of CRISPR/Cas9 (R780) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate. Lane2:HEK293T transfected with CRISPR/Cas9(L:P=3:1). Lane3:HEK293T transfected with CRISPR/Cas9(L:P=6:1). Lane4:HEK293T transfected with CRISPR/Cas9(L:P=9:1).