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NBR1 polyclonal antibody

货号: BS8985

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS8985

Product Name :NBR1 polyclonal antibody

Swiss-Prot :Q14596

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IHC, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100|IHC,1:50 - 1:200| IP, 1:50 - 1:200

Background :The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled-coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates. This gene is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of this gene results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :120KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NBR1;1A1-3B;IAI3B;M17S2;MIG19

Immunogen :Recombinant fusion protein of human NBR1(NP_005890.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using NBR1 antibody at 1:410 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunofluorescence analysis of NIH/3T3 cells using NBR1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using NBR1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.