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PARP3 polyclonal antibody

货号: BS8820

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS8820

Product Name :PARP3 polyclonal antibody

Swiss-Prot :Q9Y6F1

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100

Background :The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :60kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PARP3;ADPRT3;ADPRTL2;ADPRTL3;ARTD3;IRT1;PADPRT-3

Immunogen :Recombinant fusion protein of human PARP3(NP_001003931.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat liver, using PARP3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of MCF-7 cells using PARP3 antibody . Blue: DAPI for nuclear staining.