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ITLN1 polyclonal antibody

货号: BS8792

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS8792

Product Name :ITLN1 polyclonal antibody

Swiss-Prot :Q8WWA0

Host :Rabbit

Reactivity :Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :Intelectin-1(ITLN1) is a secreted protein and contains 1 fibrinogen C-terminal domain. Intelectin-1 is a 40 kDaCa-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed on multiple cell typesand appears to participate in insulin signaling and microbe recognition. The protein has no effect on basalglucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. It increases AKT phosphorylationin the absence and presence of insulin and it may play a role in the defense systemagainst microorganisms. Italso may specifically recognize carbohydrate chains of pathogens and bacterial components containinggalactofuranosy l residues, in a calcium-dependent manner.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :37kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ITLN1;HL-1;HL1;INTL;ITLN;LFR;hIntL;omentin

Immunogen :Recombinant fusion protein of human ITLN1(NP_060095.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using ITLN1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse intestin using ITLN1 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of rat intestine using ITLN1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse colon using ITLN1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.