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IDH2 polyclonal antibody

货号: BS8764

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS8764

Product Name :IDH2 polyclonal antibody

Swiss-Prot :P48735

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC, IP, ChIP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:20 - 1:50|IP,1:50 - 1:200|ChIP,1:20 - 1:100

Background :Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :43KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :IDH2;D2HGA2;ICD-M;IDH;IDHM;IDP;IDPM;mNADP-IDH

Immunogen :Recombinant fusion protein of human IDH2(NP_002159.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Jurkat, using IDH2 antibody at 1:650 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Western blot analysis of various lysates, using IDH2 antibody at 1:650 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human liver cancer using IDH2 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of C6 cells using IDH2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.