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GRASP65 polyclonal antibody

货号: BS8293

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS8293

Product Name :GRASP65 polyclonal antibody

Swiss-Prot :Q9BQQ3

Host :Rabbit

Reactivity :Human

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:10 - 1:100

Background :The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :65kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GORASP1;GOLPH5;GRASP65;P65

Immunogen :Recombinant fusion protein of human GRASP65(NP_114105.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using GRASP65 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of A549 cells using GRASP65 antibody . Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U2OS cells using GRASP65 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.