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POLE3 polyclonal antibody

货号: BS8241

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS8241

Product Name :POLE3 polyclonal antibody

Swiss-Prot :Q9NRF9

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB IF

Application_all :WB: 1:500~1:2000 IF: 1:50~1:200

Background :DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators . CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex),which increases the accessibility of nucleosomal DNA in an ATP-dependent manner . Unlike other known chromatin remodelling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing . This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array . In the presence of CHRAC, the nucleosomal ATPase ISWI catalyses several ATP-dependent transitions of chromatin structure .

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :POLE3 polyclonal antibody detects endogenous levels of POLE3 protein.

BiowMW :~ 20-25 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DNA polymerase epsilon subunit 3; Arsenic-transactivated protein; AsTP; Chromatin accessibility complex 17 kDa protein; CHRAC-17; HuCHRAC17; DNA polymerase II subunit 3; DNA polymerase epsilon subunit p17; POLE3; CHRAC17

Immunogen :Recombinant full length Human POLE3.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of POLE3 antibody.

Immunofluorescence analysis of U2OS cell using POLE3 antibody.

Immunofluorescence analysis of U2OS cell using POLE3 antibody. Green:gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.

Western blot analysis of extracts from normal (control) and POLE3 knockout (KO) 293T cells, using POLE3 antibody.