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ZWINT polyclonal antibody

货号: BS8135

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS8135

Product Name :ZWINT polyclonal antibody

Swiss-Prot :O95229

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :39kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ZWINT;HZwint-1;KNTC2AP;SIP30;ZWINT1

Immunogen :Recombinant fusion protein of human ZWINT(NP_008988.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using ZWINT antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of C6 cells using ZWINT Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using ZWINT Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using ZWINT Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.