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DHRS9 polyclonal antibody

货号: BS8131

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS8131

Product Name :DHRS9 polyclonal antibody

Swiss-Prot :Q9BPW9

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:10 - 1:100

Background :This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :35kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DHRS9;3-alpha-HSD;3ALPHA-HSD;RDH-E2;RDH-TBE;RDH15;RDHL;RDHTBE;RETSDR8;SDR9C4

Immunogen :Recombinant fusion protein of human DHRS9(NP_001135743.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using DHRS9 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit ._Exposure time: 390s.

Western blot analysis of extracts of various cell lines, using DHRS9 antibody at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.

Immunofluorescence analysis of U2OS cells using DHRS9 antibody . Blue: DAPI for nuclear staining.