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ATGL/PNPLA2 polyclonal antibody

货号: BS7989

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS7989

Product Name :ATGL/PNPLA2 polyclonal antibody

Swiss-Prot :Q96AD5

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :55kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PNPLA2;1110001C14Rik;ATGL;FP17548;PEDF-R;TTS-2.2;TTS2;iPLA2zeta

Immunogen :Recombinant fusion protein of human PNPLA2(NP_065109.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using ATGL/PNPLA2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.

Immunofluorescence analysis of U2OS cells using ATGL/PNPLA2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats

JournalJournal Of Nutritional Biochemistry

ApplicationWB

IF4.49

SpeciesRat

PMID
miR-103 promotes 3T3-L1 cell adipogenesis through AKT/mTOR signal pathway with its target being MEF2D

JournalBiological Chemistry

ApplicationWB

IF3.915

SpeciesMouse

PMID25400071
Chronic dexamethasone exposure markedly decreased the hepatic triglyceride accumulation in growing goats

JournalGeneral And Comparative Endocrinology

ApplicationWB

IF2.445

SpeciesGoat

PMID29155266
Butyrate stimulates adipose lipolysis and mitochondrial oxidative phosphorylation through histone hyperacetylation‐associated β3‐adrenergic receptor activation in high‐fat diet‐induced obese mice

JournalExperimental Physiology

ApplicationWB

IF2.431

SpeciesMouse

PMID28028849
Alpha-Lipoic Acid Alleviates Acute Inflammation and Promotes Lipid Mobilization During the Inflammatory Response in White Adipose Tissue of Mice

JournalLipids

ApplicationWB

IF1.88

SpeciesMouse

PMID27531126