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SCNN1G polyclonal antibody

货号: BS7921

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7921

Product Name :SCNN1G polyclonal antibody

Swiss-Prot :P51170

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:100

Background :Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :80kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SCNN1G;BESC3;ENaCg;ENaCgamma;PHA1;SCNEG

Immunogen :Recombinant fusion protein of human SCNN1G(NP_001030.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using SCNN1G antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.

Immunohistochemistry of paraffin-embedded mouse lung using SCNN1G antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.