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WNT2 polyclonal antibody

货号: BS7909

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS7909

Product Name :WNT2 polyclonal antibody

Swiss-Prot :P09544

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :56kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :WNT2;INT1L1;IRP

Immunogen :Recombinant fusion protein of human WNT2(NP_003382.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of SKOV3 cells, using WNT2 Antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat brain using WNT2 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human liver damage using WNT2 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Down-regulation of miR-30a-3p/5p promotes esophageal squamous cell carcinoma cell proliferation by activating the Wnt signaling pathway

JournalWorld Journal Of Gastroenterology

ApplicationWB

IF3.665

SpeciesHuman

PMID29259372
Multi-faceted epigenetic dysregulation of gene expression promotes esophageal squamous cell carcinoma

JournalNature Communications

ApplicationIHC

IF12.121

SpeciesHuman

PMID32699215
GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer

JournalCell Death & Disease

ApplicationWB

IF8.469

SpeciesHuman

PMID34671013