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TGM3 polyclonal antibody

货号: BS7902

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7902

Product Name :TGM3 polyclonal antibody

Swiss-Prot :Q08188

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:10 - 1:100

Background :Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :77KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TGM3;TGE;UHS2

Immunogen :Recombinant fusion protein of human TGM3(NP_003236.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of A-549, using TGM3 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 0.5s.

Immunofluorescence analysis of U2OS cells using TGM3 antibody . Blue: DAPI for nuclear staining.