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KDM4B/JMJD2B polyclonal antibody

货号: BS7882

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7882

Product Name :KDM4B/JMJD2B polyclonal antibody

Swiss-Prot :O94953

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :150KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :KDM4B;JMJD2B;TDRD14B

Immunogen :Recombinant fusion protein of human KDM4B/JMJD2B(NP_055830.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Mouse brain, using at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.