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ATG3 polyclonal antibody（KU）

货号: BS7872

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS7872

Product Name :ATG3 polyclonal antibody（KU）

Swiss-Prot :Q9NT62

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB IP

Application_all :WB,1:500 - 1:2000 IP: 1:50~1:200

Background :This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ATG3 polyclonal antibody detects endogenous levels of ATG3 protein.

BiowMW :~ 40 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ATG3; APG3; APG3-LIKE; APG3L; PC3-96

Immunogen :Recombinant fusion protein of human ATG3.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of ATG3 pAb at 1:2000 dilution Lane1:K562 whole cell lysate(30ug) Lane2:THP-1 whole cell lysate(30ug) Lane3:BV2 whole cell lysate(30ug) Lane4:PC12 whole cell lysate(30ug)

Immunoprecipitation of PC12 cell lysates using ATG3 pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using ATG3 pAb.

Sodium tanshinone IIA sulfonate protects ARPE-19 cells against oxidative stress by inhibiting autophagy and apoptosis

JournalScientific Reports

ApplicationWB

IF4.011

SpeciesHuman

PMID30310136