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PP2A-Aα/PR65α/PPP2R1A polyclonal antibody

货号: BS7866

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

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No. :BS7866

Product Name :PP2A-Aα/PR65α/PPP2R1A polyclonal antibody

Swiss-Prot :P30153

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:10 - 1:100

Background :This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :65KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PPP2R1A;MRD36;PP2A-Aalpha;PP2AA;PP2AAALPHA;PR65A

Immunogen :Recombinant fusion protein of human PP2A-Aalpha/PR65α/PP2A-Aα/PR65α/PPP2R1A(NP_055040.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using PP2A-Aalpha/PR65α/PP2A-Aα/PR65α/PPP2R1A Polyclonal Antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of U2OS cells using PP2A-Aalpha/PR65α/PP2A-Aα/PR65α/PPP2R1A antibody . Blue: DAPI for nuclear staining.