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CD14 polyclonal antibody

货号: BS7825

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS7825

Product Name :CD14 polyclonal antibody

Swiss-Prot :P08571

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :60KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CD14

Immunogen :Recombinant fusion protein of human CD14(NP_001167576.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of RAW264.7 cells, using CD14 antibody at 1:608 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of RAW264.7 cells using CD14 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of THP-1 cells using CD14 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.