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CRYAA polyclonal antibody

货号: BS7816

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7816

Product Name :CRYAA polyclonal antibody

Swiss-Prot :P02489

Host :Rabbit

Reactivity :Human, Mouse, Rat, Zebrafish

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :19KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CRYAA;CRYA1;CTRCT9;HSPB4

Immunogen :Recombinant fusion protein of human CRYAA(NP_000385.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of HepG2, using CRYAA antibody at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of Rat Eye using CRYAA Rabbit pAb at dilution of 1:200 . Blue: DAPI for nuclear staining.

Lanosterol and 25-hydroxycholesterol dissociate crystallin aggregates isolated from cataractous human lens via different mechanisms

JournalBiochemical And Biophysical Research Communications

ApplicationWB

IF3.575

SpeciesHuman

PMID30392915