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PMEL polyclonal antibody

货号: BS7760

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS7760

Product Name :PMEL polyclonal antibody

Swiss-Prot :P40967

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :80KDa/105KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PMEL;D12S53E;ME20;ME20-M;ME20M;P1;P100;PMEL17;SI;SIL;SILV;gp100

Immunogen :Recombinant fusion protein of human PMEL(NP_008859.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of A375 cells, using PMEL antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3min.

Immunofluorescence analysis of A375 cells using PMEL Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.