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APTX polyclonal antibody

货号: BS7755

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7755

Product Name :APTX polyclonal antibody

Swiss-Prot :Q7Z2E3

Host :Rabbit

Reactivity :Human

Applications :WB, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100|IP,1:20 - 1:50

Background :This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :40kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :APTX;AOA;AOA1;AXA1;EAOH;EOAHA;FHA-HIT;aprataxin

Immunogen :Recombinant fusion protein of human APTX(NP_001182178.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using APTX antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of A549 cells using APTX antibody .

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug APTX antibody . Western blot was performed from the immunoprecipitate using APTX antibody at a dilution of 1:500.