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GST3/GSTP1 polyclonal antibody（KU）

货号: BS7629

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS7629

Product Name :GST3/GSTP1 polyclonal antibody（KU）

Swiss-Prot :P09211

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :25kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DFN7;FAEES3;GST3;GSTP;HEL-S-22;PI;GST3 / GSTP1;GSTP1

Immunogen :Recombinant fusion protein of human GST3/GST3/GSTP1(NP_000843.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using GST3/GST3/GSTP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human liver cancer using GST3/GST3/GSTP1 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse kidney using GST3/GST3/GSTP1 antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of L929 cells using GST3/GST3/GSTP1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Glutathione S-transferases P1 protects breast cancer cell from adriamycin-induced cell death through promoting autophagy

JournalCell Death And Differentiation

ApplicationWB,IF

IF8.086

SpeciesHuman

PMID30683915
Glutathione S-Transferase Pi Prevents Sepsis-Related High Mobility Group Box-1 Protein Translocation and Release

JournalFrontiers in Immunology

ApplicationWB,PULL DOWN,IP

IF5.085

SpeciesMouse,Human

PMID29520271
Glutathione S-transferases P1-mediated interleukin-6 in tumor-associated macrophages augments drug-resistance in MCF-7 breast cancer

JournalBiochemical Pharmacology

ApplicationWB

IF4.96

SpeciesHuman, Mouse

PMID33080187