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L-Plastin/LCP1 polyclonal antibody

货号: BS7623

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS7623

Product Name :L-Plastin/LCP1 polyclonal antibody

Swiss-Prot :P13796

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:20 - 1:50

Background :Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :70kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LCP1;CP64;HEL-S-37;L-PLASTIN;LC64P;LPL;PLS2;plastin-2

Immunogen :Recombinant fusion protein of human L-Plastin/LCP1(NP_002289.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using L-Plastin/LCP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug L-Plastin/LCP1 antibody . Western blot was performed from the immunoprecipitate using L-Plastin/LCP1 antibody at a dilution of 1:1000.