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Myoglobin polyclonal antibody

货号: BS7593

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS7593

Product Name :Myoglobin polyclonal antibody

Swiss-Prot :P02144

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :17KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MB;PVALB;myoglobgin;myoglobin

Immunogen :RecoMyoglobin inant fusion protein containing a sequence corresponding to amino acids 1-1-154 of human Myoglobin (NP_005359.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using Myoglobin antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunofluorescence analysis of rat skeletal muscle cells using Myoglobin Rabbit pAb at dilution of 1:300 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse skeletal muscle cells using Myoglobin Rabbit pAb at dilution of 1:300 . Blue: DAPI for nuclear staining.