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SSCA1/SerpinB3 polyclonal antibody

货号: BS7581

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

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No. :BS7581

Product Name :SSCA1/SerpinB3 polyclonal antibody

Swiss-Prot :P29508

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :Serpin B3, also known as squamous cell carcinoma antigen-1 (SCCA-1), is a member of the serpin superfamily of serine protease inhibitors. Serpin B3 belongs to the subgroup ovalbumin-related serpins which are involved in the regulation of apoptosis, inflammation, angiogenesis and embryogenesis. It may act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. It also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :45KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SERPINB3;HsT1196;SCC;SCCA-1;SCCA-PD;SCCA1;SSCA1;T4-A;serpin B3

Immunogen :Recombinant fusion protein of human SSCA1/SerpinB3(NP_008850.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of 293T, using SSCA1/SerpinB3 antibody at 1:1020 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of A431 cells using SSCA1/SerpinB3 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HepG2 cells using SSCA1/SerpinB3 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.