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NOTCH3 polyclonal antibody

货号: BS7500
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS7500
Product Name :NOTCH3 polyclonal antibody
Swiss-Prot :Q9UM47
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IF/ICC
Application_all :WB,1:200 - 1:1000|IF/ICC,1:50 - 1:200
Background :This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :90kDa/280kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :NOTCH3;CADASIL;CADASIL1;CASIL;IMF2;LMNS;notch 3
Immunogen :Recombinant fusion protein of human NOTCH3(NP_000426.2).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of 293T cells, using NOTCH3 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 90s.
Immunofluorescence analysis of NIH/3T3 cells using NOTCH3 Rabbit pAb at dilution of 1:200 . Blue: DAPI for nuclear staining.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com