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CX3CR1 polyclonal antibody

货号: BS7449

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7449

Product Name :CX3CR1 polyclonal antibody

Swiss-Prot :P49238

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :52KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CX3CR1;CCRL1;CMKBRL1;CMKDR1;GPR13;GPRV28;V28

Immunogen :A synthetic peptide of human CX3CR1(NP_001328.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using CX3CR1 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using CX3CR1 Rabbit pAb at dilution of 1:25 .Perform microwave antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat spleen using CX3CR1 Rabbit pAb at dilution of 1:25 .Perform microwave antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.