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NEUROG3 polyclonal antibody

货号: BS7437

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7437

Product Name :NEUROG3 polyclonal antibody

Swiss-Prot :Q9Y4Z2

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:100

Background :The protein encoded by this gene is a basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of congenital malabsorptive diarrhea 4 (DIAR4).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :25KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NEUROG3;Atoh5;Math4B;NGN-3;bHLHa7;ngn3

Immunogen :Recombinant fusion protein of human NEUROG3(NP_066279.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using NEUROG3 antibody at 1:1730 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human colon using NEUROG3 Rabbit pAb at dilution of 1:250 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.