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EGF polyclonal antibody

货号: BS7426
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS7426
Product Name :EGF polyclonal antibody
Swiss-Prot :P01133
Host :Rabbit
Reactivity :Human, Mouse
Applications :DB, WB
Application_all :DB,1:500 - 1:2000|WB,1:500 - 1:2000
Background :This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :160kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :EGF;HOMG4;URG
Immunogen :Recombinant fusion protein of human EGF(NP_001954.2).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of mouse kidney, using EGF antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
Western blot analysis of extracts of 293T cells, using EGF antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
Dot-blot analysis of purified mature EGF using EGF antibodyat 1:500 dilution。

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com