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Phospho-RPS6KA5-S376 polyclonal antibody

货号: BS74019

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS74019

Product Name :Phospho-RPS6KA5-S376 polyclonal antibody

Swiss-Prot :O75582

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100

Background :Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :90kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RPS6KA5;MSK1;MSPK1;RLPK

Immunogen :A synthetic phosphorylated peptide around S376 of human RPS6KA5(NP_004746.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-RPS6KA5-S376 antibody at 1:2000 dilution. NIH/3T3 cells were treated by Anisomycin for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat testis using Phospho-RPS6KA5-S376 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-RPS6KA5-S376 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-RPS6KA5-S376 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.