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Acetyl-Histone H4-K8 polyclonal antibody

货号: BS74016

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS74016

Product Name :Acetyl-Histone H4-K8 polyclonal antibody

Swiss-Prot :P62805

Host :Rabbit

Reactivity :Human, Mouse, Rat, Other (Wide Range)

Applications :WB, IHC, IF/ICC, IP, ChIP, ChIP-seq

Background :Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Acetylated Antibodies

BiowMW :11kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :FO108;H4;H4/n;H4F2;H4FN;HIST2H4;Histone H4;HIST1H4A;HIST2H4A

Immunogen :A synthetic acetylated peptide around K8 of human Histone H4(NP_003529.1).

Conjugate :Unconjugated

Modification :Acetylated

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K8 antibody at 1:1000 dilution.NIH/3T3 cells and C6 cells were treated by TSA at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human kidney using Acetyl-Histone H4-K8 Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse liver using Acetyl-Histone H4-K8 Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat kidney using Acetyl-Histone H4-K8 Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

RNF8 promotes efficient DSB repair by inhibiting the pro‐apoptotic activity of p53 through regulating the function of Tip60

JournalCell Proliferation

ApplicationWB

IF5.753

SpeciesHuman

PMID32031738
Histone acetylation by HBO1 (KAT7) activates Wnt/β-catenin signaling to promote leukemogenesis in B-cell acute lymphoblastic leukemia

JournalCell Death & Disease

ApplicationWB

IF9

SpeciesHuman，Mouse

PMID37542030