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Acetyl-Histone H3-K18 polyclonal antibody

货号: BS74015

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS74015

Product Name :Acetyl-Histone H3-K18 polyclonal antibody

Swiss-Prot :P68431

Host :Rabbit

Reactivity :Human, Mouse, Rat, Other (Wide Range)

Applications :WB, IHC, IF/ICC, IP, ChIP, ChIP-seq

Background :Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Acetylated Antibodies

BiowMW :17KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HIST1H3J;H3/j;H3FJ

Immunogen :A synthetic acetylated peptide around K18 of human H3(NP_003520.1).

Conjugate :Unconjugated

Modification :Acetylated

Western blot analysis of extracts of HeLa cells, using Acetyl-Histone H3-K18 antibody at 1:500 dilution.HeLa cells were treated by TSA at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat brain using H3K18ac antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human gastric cancer using H3K18ac antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

NOK mediates glycolysis and nuclear PDC associated histone acetylation

JournalFrontiers in Bioscience-Landmark

ApplicationWB

IF4.009

SpeciesMouse

PMID28410146