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TAB1 polyclonal antibody

货号: BS7256

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS7256

Product Name :TAB1 polyclonal antibody

Swiss-Prot :Q15750

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:10 - 1:100|IP,1:50 - 1:200

Background :The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :70kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TAB1;3'-Tab1;MAP3K7IP1

Immunogen :Recombinant fusion protein of human TAB1(NP_006107.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using TAB1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of U2OS cells using TAB1 antibody . Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug TAB1 antibody . Western blot was performed from the immunoprecipitate using TAB1 antibody at a dilution of 1:1000.