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LIN28A polyclonal antibody

货号: BS72356
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS72356
Product Name :LIN28A polyclonal antibody
Swiss-Prot :Q9H9Z2
Host :Rabbit
Reactivity :Human, Mouse
Applications :WB, IHC, IF/ICC
Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200
Background :This gene encodes a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells. The encoded protein functions through direct interaction with target mRNAs and by disrupting the maturation of certain miRNAs involved in embryonic development. This protein prevents the terminal processing of the LET7 family of microRNAs which are major regulators of cellular growth and differentiation. Aberrant expression of this gene is associated with cancer progression in multiple tissues.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :28KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :LIN28A;CSDD1;LIN-28;LIN28;ZCCHC1;lin-28A
Immunogen :A synthetic peptide of human LIN28A(NP_078950.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of T47D cells, using LIN28A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded Human testicular seminoma using NY-ESO-1 Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com