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SHP2 polyclonal antibody

货号: BS7221
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS7221
Product Name :SHP2 polyclonal antibody
Swiss-Prot :Q06124
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IF/ICC
Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100
Background :The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :72KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :BPTP3;CFC;JMML;METCDS;NS1;PTP-1D;PTP2C;SH-PTP2;SH-PTP3;SHP2;PTPN11
Immunogen :Recombinant fusion protein of human SHP2(NP_001317366.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using SHP2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat heart using SHP2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human gastric cancer using SHP2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse spleen using SHP2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • SHP-2 Promotes the Maturation of Oligodendrocyte Precursor Cells Through Akt and ERK1/2 Signaling In Vitro
    JournalPLoS One
    ApplicationWB,IHC
    IF3.24
    SpeciesRat
    PMID
  • Bacteroides fragilis alleviates the symptoms of lupus nephritis via regulating CD1d and CD86 expressions in B cells
    JournalEuropean Journal Of Pharmacology
    ApplicationWB
    IF3.263
    SpeciesMouse

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com