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RAD17 polyclonal antibody

货号: BS7210

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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No. :BS7210

Product Name :RAD17 polyclonal antibody

Swiss-Prot :O75943

Host :Rabbit

Reactivity :Human

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:200

Background :The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :77kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RAD17;CCYC;HRAD17;R24L;RAD17SP;RAD24

Immunogen :Recombinant fusion protein of human RAD17(NP_002864.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using RAD17 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug RAD17 antibody . Western blot was performed from the immunoprecipitate using RAD17 antibody at a dilution of 1:1000.