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PSMB6 polyclonal antibody

货号: BS71993

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71993

Product Name :PSMB6 polyclonal antibody

Swiss-Prot :P28072

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100

Background :The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :22KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PSMB6;DELTA;LMPY;Y

Immunogen :Recombinant fusion protein of human PSMB6(NP_002789.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using PSMB6 antibody at 1:1350 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of HeLa cells using PSMB6 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.