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RNH1 polyclonal antibody

货号: BS71992

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71992

Product Name :RNH1 polyclonal antibody

Swiss-Prot :P13489

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:1000 - 1:2000|IF/ICC,1:50 - 1:200

Background :Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :43kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RNH1;RAI;RNH

Immunogen :Recombinant fusion protein of human RNH1(NP_002930.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using RNH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of C6 cells using RNH1 Rabbit pAb at dilution of 100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using RNH1 Rabbit pAb at dilution of 100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using RNH1 Rabbit pAb at dilution of 100 . Blue: DAPI for nuclear staining.