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PIAS2 polyclonal antibody

货号: BS7196

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7196

Product Name :PIAS2 polyclonal antibody

Swiss-Prot :O75928

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :75kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PIAS2;ARIP3;DIP;MIZ;MIZ1;PIASX;PIASX-ALPHA;PIASX-BETA;SIZ2;ZMIZ4

Immunogen :Recombinant fusion protein of human PIAS2(NP_004662.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of mouse testis, using PIAS2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunohistochemistry of paraffin-embedded mouse brain using PIAS2 Rabbit pAb at dilution of 1:25 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.