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BACE1 polyclonal antibody

货号: BS71952

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71952

Product Name :BACE1 polyclonal antibody

Swiss-Prot :P56817

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :70KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BACE1;ASP2;BACE;HSPC104

Immunogen :Recombinant fusion protein of human BACE1(NP_036236.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of SH-SY5Y, using BACE1 antibody at 1:582 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse hippocampus using BACE1 Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse kidney using BACE1 Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of U-251MG cells using BACE1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

SOCE-mediated NFAT1–NOX2–NLRP1 inflammasome involves in lipopolysaccharide-induced neuronal damage and Aβ generation

JournalMolecular Neurobiology

ApplicationWB

IF5.59

SpeciesMouse

PMID35286582
Chronic glucocorticoid exposure accelerates Aβ generation and neurotoxicity by activating calcium-mediated CN-NFAT1 signaling in hippocampal neurons in APP/PS1 mice

JournalFOOD AND CHEMICAL TOXICOLOGY

ApplicationWB

IF5.572

SpeciesMouse

PMID36075474