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GNRH1 polyclonal antibody

货号: BS71943

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71943

Product Name :GNRH1 polyclonal antibody

Swiss-Prot :P01148

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a preproprotein that is proteolytically processed to generate a peptide that is a member of the gonadotropin-releasing hormone (GnRH) family of peptides. Alternative splicing results in multiple transcript variants, at least one of which is secreted and then cleaved to generate gonadoliberin-1 and GnRH-associated peptide 1. Gonadoliberin-1 stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. Mutations in this gene are associated with hypogonadotropic hypogonadism.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :12KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GNRH1;GNRH;GRH;LHRH;LNRH

Immunogen :Recombinant fusion protein of human GNRH1(NP_001076580.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Mouse lung, using GNRH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Western blot analysis of extracts of Rat kidney, using GNRH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 60s.

Immunofluorescence analysis of SH-SY5Y cells using GNRH1 Rabbit pAb at dilution of 1:200 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using GNRH1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.