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ErbB4/HER4 polyclonal antibody

货号: BS71932

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS71932

Product Name :ErbB4/HER4 polyclonal antibody

Swiss-Prot :Q15303

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100

Background :This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :180kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ALS19;HER4;p180erbB4;ERBB4;HER4/ErbB4

Immunogen :A synthetic peptide of human ErbB4/HER4(NP_005226.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of mouse brain, using ErbB4/HER4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human placenta using ErbB4/HER4 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of C6 cells using ErbB4/HER4 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using ErbB4/HER4 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.