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Leptin Receptor polyclonal antibody

货号: BS7184

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7184

Product Name :Leptin Receptor polyclonal antibody

Swiss-Prot :P48357

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:1000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :140KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CD295;LEP-R;LEPRD;OB-R;OBR;LEPR

Immunogen :Recombinant fusion protein of human LEPR(NP_002294.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Leptin Receptor antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of HepG2 cells using Leptin Receptor Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Leptin enhances the invasive ability of glioma stem-like cells depending on leptin receptor expression

JournalBrain Research

ApplicationWB

IF2.733

SpeciesHuman

PMID
Plumbagin Inhibits Leptin-Induced Proliferation of Hepatic Stellate Cells via JAK2-STAT3 Pathway to Protect against Hepatic Fibrosis

JournalTropical Journal Of Pharmaceutical Research

ApplicationWB

IF0.504

SpeciesHuman

PMID