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METTL4 polyclonal antibody

货号: BS71800

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71800

Product Name :METTL4 polyclonal antibody

Swiss-Prot :Q8N3J2

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:1000 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :N(6-adenine-specific methyltransferase that can methylate both RNAs and DNA. Acts as a N(6-adenine-specific RNA methyltransferase by catalyzing formation of N6,2'-O-dimethyladenosine (m6A(m on internal positions of U2 small nuclear RNA (snRNA: methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation. Internal m6A(m methylation of snRNAs regulates RNA splicing. Also able to act as a N(6-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N(6-methyladenosine. The existence of N(6-methyladenosine (m6A on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N(6-adenine-specific DNA methyltransferase activity of METTL4 in vivo. Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA copy number by mediating mtDNA N(6-methylation: m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending. N(6-methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing (By similarity.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :54kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :METTL4;HsT661

Immunogen :Recombinant fusion protein of human METTL4(NP_073751.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using METTL4 antibody at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.