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RDH5 polyclonal antibody

货号: BS71604

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS71604

Product Name :RDH5 polyclonal antibody

Swiss-Prot :Q92781

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100

Background :This gene encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream BLOC1S1 (biogenesis of lysosomal organelles complex-1, subunit 1) gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :38kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RDH5;9cRDH;HSD17B9;RDH1;SDR9C5

Immunogen :Recombinant fusion protein of human RDH5(NP_002896.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using RDH5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 90s.

Immunofluorescence analysis of rat retina using RDH5 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse retina using RDH5 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.