Western blot analysis of STRAD alpha expression in Jurkat (A) whole cell lysates.
Immunofluorescent analysis of STRAD alpha staining in A549 cells.
Flow cytometric analysis of A549 cells using Anti-STRAD alpha Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
