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ARHGAP1 polyclonal antibody

货号: BS71523

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS71523

Product Name :ARHGAP1 polyclonal antibody

Swiss-Prot :Q07960

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:100

Background :This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :50kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ARHGAP1;CDC42GAP;RHOGAP;RHOGAP1;p50rhoGAP

Immunogen :Recombinant fusion protein of human ARHGAP1(NP_004299.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using ARHGAP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 15s.

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug ARHGAP1 antibody . Western blot was performed from the immunoprecipitate using ARHGAP1 antibody at a dilution of 1:1000.