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GTF2H1 polyclonal antibody

货号: BS71475

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS71475

Product Name :GTF2H1 polyclonal antibody

Swiss-Prot :P32780

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:1000|IF/ICC,1:50 - 1:200

Background :Component of the general transcription and DNA repair factor IIH (TFIIH core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :65kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GTF2H1;BTF2;P62;TFB1;TFIIH

Immunogen :Recombinant fusion protein of human GTF2H1(NP_001135779.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using GTF2H1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of U-2 OS cells using GTF2H1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.